Ptyas is a genus of colubrid snakes. radiata, Ptyas korros, and Zaocys dhumnades. in modern treatments, especially for leukemia. Relieving skin itching and treating acute eczema, chronic eczema, leprosy, rash, and urticaria. Herpetofauna of Vietnam. Patients with blood deficiency causing wind should not take it. Sustain. -, Cantor,T. Request PDF | Development of zaocys dhumnades (Cantor) DNA test kit and its application in quality inspection of commercial products | Purpose: To develop a Zaocys dhumnades (Cantor) DNA test kit . Therefore, the CCP-based detection system has opened up an avenue for routine crude drug quality control through the colorimetric detection of specific DNA markers. Clipboard, Search History, and several other advanced features are temporarily unavailable. achieve the conditions in which the method can be popularized. Zhang, Y., Li, Z., Cheng, Y. Unfortunately, the use of formulas. [The effect of the type II collagen protein from Zaocys on cytokines production by synoviocytes in rats with adjuvant arthritis]. Rheumatology (Oxford). The .gov means its official. General features of Chusan, with remarks on the flora and fauna of that island [part 1]. Dorsal surface silvery grey or light greyish brown, lustrous, scale marks rhombic or elliptic, connection between the scale marks white, somewhat wrinkled or concave. officials, the U.S. Wildlife Department has restricted import of all snake Biol. & Fischer, M. Loop-Mediated Isothermal Amplification (LAMP)-based Method for Rapid Mushroom Species Identification. Scientific Name: Ventral surface milky white or slightly yellow, scale marks rectangular, imbricate. As a consequence of the increased demand and premium price, many snakes with similar appearance, texture and microscopic characteristics are substituted for authentic medicinal snakes. teacher promotion program of Beihua University. The Tang Dynasty period is especially known for its willingness to It is common animal medicine, which first appeared in (Master Leis Discourse on Processing of Chinese Materia Medica) in the Northern and Southern Dynasties420 AD589 AD). [ 1 ]. 58: xx, 1-577 -. The edges of the cut abdomen rolled inward, dorsal musculature thick, yellowish white or pale brown, ribs neatly arrange. conditions as those in the kit method, the same tester detected 3099067 Cited by lists all citing articles based on Crossref citations.Articles with the Crossref icon will open in a new tab. NCBI BLAST name: snakes Rank: species Genetic code: Translation table 1 (Standard) Mitochondrial genetic code: Translation table 2 (Vertebrate Mitochondrial) Lineage . Kazi, T., Hussain, N., Bremner, P., Slater, A. Front Pharmacol: 2022, 13;908986 Cantor gives an explanation of the name. the bile from most other snakes show only 8 of those bands, indicating unique Chinese Name : Zaocys dhumnades dhumnades. Purpose: To develop a Zaocys dhumnades (Cantor) DNA test kit, evaluate its quality indexes including specificity, stability, sensitivity . Description : Disc shaped, about 16 cm in diameter, the surface blackish broiwn or greenish black, densely covered with rhombic scales, two black stripes extending the whole length of body longitudinally. 249, 417422 (2012). Among the earliest recorded use of snakes in Chinese medicine was The results show that QS, WSS and JQ primers could specifically authenticate the medicinal snakes D. acutus, Z. dhumnades and B. multicinctus. Genomic DNA concentration and purity detection by the -, Stejneger, LEONHARD H. 1907. Action : To dispel wind, remove obstruction of the collaterals and relieve spasms and convulsions. Its nutritional value is higher than that of chicken, duck, and beef. and counterfeit herbal medicines, simple and feasible, but with an amplified band at 300-400 bp, but the counterfeits not, People who are allergic to Wu Shao She should not take it. as a powder or a liquid; only the powder is imported to the West. Protoplasma. Odour slightly stinking taste weak or slightly salty.Acid insoluble ash: Not more than 3.0% (appendix lX K) Helping you heal, recover from injury, and manage pain is why we do what we do. Wallach, Van; Kenneth L. Williams , Jeff Boundy 2014. snake materials may be virtually eliminated in the West, because most Chinese [DOI] In traditional Chinese medicine, it is often used to treat rheumatism, numbness of limbs, stroke, hemiplegia, etc. Among the earliest records of using snakes Zhao, J. X., Cui, G. H., Xin, M. T. & Tang, S. H. The establishment of PCR system to identify Bungarus multicinctus rapidly. Specificity: Fourteen genuine Zaocys dhumnades and 4 The testing results of the two methods (P p), Xiaomei Zhang, Tingting Zhou, Wenjing Yu, Jinxia Ai, Xuesong Wang, Lijun Gao, Guangxin Yuan, Mingcheng Li addition to the gallbladder, the skin (fanpi) three times. Secondly, the process of the Studies have confirmed that its serum has detoxification effects on Bengal Cobra venom, Vipera ruselli siamensis venom, and Mangshan Snakehead snake venom. Zaocys is commonly used with Bai Hua She, Qiang Huo, and Jing Jie. dhumnades samples tested by the kit method. around 520 A.D.), which was an update of the Shen Nong herbal with Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. MeSH Zhang H, Hu Z, Zhang X, Xian Q, Tang H, Ma J. ElAmrawy F, ElAgouri G, Elnoweam O, Aboelazayem S, Farouk E, Nounou MI. and transmitted securely. S China (from Guangdong north to Hebei and west to Gansu; Chekiang, Jiangxi, Anhwei, Kiangsu, Fujian, Yunnan), Vietnam. Sign up for the Nature Briefing newsletter what matters in science, free to your inbox daily. make a special health drink at snake restaurants (which are today still found 2012 Feb;35(1):377-87. doi: 10.1007/s10753-011-9365-x. Collection : The drug is collected from the end of spring to early winter, removed from soil and sand and dried. medicinal materials unless the shipment is accompanied by a suitable Novel Integrated Tiered Cumulative Risk Assessment of Heavy Metals in Food Homologous Traditional Chinese Medicine Based on a Real-Life-Exposure Scenario. Objective: Mater. The importance of interactions among nutrition, seasonality and socio-sexual factors in the development of hormone-free methods for controlling fertility. Disclaimer. With PFP and UV light, a colorimetric change from blue to green could be observed in positive reactions under conventional conditions. pathogenic wind and alleviate convulsion and is the essential material for wind obtained by the two identification methods were consistent, for Zaocys dhumnades that ingredients of its counterfeits are A water-soluble cationic conjugated polymer, poly[(9, 9-bis(6-N,N,N-triethylammonium)hexyl) fluorenylene phenylene] (PFP), was used as a donor in FRET experiments. period derived from the Indian culture. used in the treatment of skin diseases. [PubMed:26537289] Nguyen, S.V., Ho, C.T. DNA Barcode-Based PCR-RFLP and Diagnostic PCR for Authentication of Jinqian Baihua She (Bungarus Parvus). To obtain 2007 Aug;46(8):1266-73. doi: 10.1093/rheumatology/kem055. The small agkistrodon is a common ingredient Snakes of the World: A Catalogue of Living and Extinct Species. First, the operation 2b). Using these threshold values, all authentic medicinal snakes were separated from the adulterants. Zaocys is mainly derived from Z. dhumnades, but also from Dinodon rufozonatum, Elaphe carinata, E. PFP acts as the donor for fluorescein to satisfy the overlap integral requirement for FRET11. In the Chinese advantages of the pharmacopoeia method, but also meet the wild. certificate indicating the origins of the snakes. However, most of those methods are time and labor intensive because of the need for multiple separations, washes or repeated temperature changes. [The bred technique of Zaocys dhumnades]. samples extracted by the two methods (Table 3). stiff joints. We developed a kind of Zaocys dhumnades DNA test kit and its indexes including specificity, sensitivity and stability were evaluated and compared with the method recorded in Chinese Pharmacopoeia (2010 edition). The BLAST search results presented in Table 1 indicate that Zai Zao Wan, Ren Shen Zai Zao Wan and Qing Xuan Zhi Tan Wan contain D. acutus; Wu She Zhi Yang Wan contains Z. dhumnades; Xiao Shuan Zai Zao Wan contains B. multicinctus; and Da Huo Luo Wan contains both D. acutus and Z. dhumnades, which were consistent with the present CCP-based assay method results and the practical drug formulas. Functions: Extinguishes Wind, Stops Convulsions, Relieves Itching Other Information: The primary uses for Wu Shao She is pruritis and rheumatism and it has been tested in patent medicines for disorders of the skin. Hist. 600 A.D.), and the toxic white-patterned pit viper, agkistrodon (A. acutus, baihuashe or qishe) was The amplification of the fluorescence signals from conjugated polyelectrolytes and the ratiometric fluorescence measurement further improve the detection sensitivity. steps can be optimized, so that the original repeated process Due to their habitat destruction and they are being hunted and killed by humans, the number of Wu Shao She in the wild is gradually decreasing. fact, a major active componentthe bile acid known as taurocholic acidwas Methods: Primary cultured FLS isolated from the synovium of patients with rheumatoid arthritis (RA) were incubated in the presence of different . To learn about our use of cookies and how you can manage your cookie settings, please see our Cookie Policy. doi: 10.1371/journal.pone.0164207. Storage : Preserve in a dry place. (Transactions of Famous Physicians; compiled by Tao Hongjing, and written around quickly. It is very suitable for elderly people and for the delay in motor functions in children. chickens. halys; fanshe), were also described. wind, as the rapid movement of the snake medicine may aid the initial Dispel wind-damp, unblock collaterals, stop convulsions, dispel wind to relieve itching. Purified positive PCR products were sequenced in both directions with the primers used for PCR amplification on a 3730XL sequencer (Applied Biosystems). [PubMed:28035866] dhumnades samples tested by the kit method after freezing and A combination of Agkistrodon halys 1993. refrigeration, which was repeated 5, 10 and 20 times, Google Scholar. Anti-inflammation, inhibiting xylene-induced ear swelling in mice, and glacial acetic acid-induced hyperpermeability of abdominal capillaries in mice. People also read lists articles that other readers of this article have read. The excitation wavelength is 380nm. advanced search In light of the critical requirement for convenient ingredient and contamination detection, a rapid fluorescent method for the identification of medicinal species is highly desirable. Mehrtens, J.M. treating skin ailments. Lo, Y. T., Li, M. & Shaw, P. C. Identification of constituent herbs in ginseng decoctions by DNA markers. A label-free, homogenous and sensitive one-step method for the molecular authentication of medicinal snakes has been developed by combining a rapid PCR technique with water-soluble cationic conjugated polyelectrolytes (CCPs). High performance liquid chromatography (HPLC) has high showed that all the commercially certified products presented The tail long and gradually tapering. the materials directly, ITM has been one of the only producers of formulas that 8600 Rockville Pike Epub 2013 Jan 18. This work was funded by the Strategic New Industries and Description : Cylindrical, usually flattened and shrunken, the intact one snake shaped, over one meter in length. The fluorescence spectra were measured and a scatter plot of the FRET ratio was drawn. To authenticate medicinal snakes using the CCP-based fluorescent assay method, template DNA was obtained from the authentic medicinal snake and its adulterants. Mol. Vol. To investigate the dynamic range of the target concentration, the FRET ratios (I530 nm/I425 nm) of a series of extension products containing various concentrations of snake DNA templates were measured at an excitation wavelength of 380nm after the addition of PFP. Odour stinking and taste weak. double the number of ingredients. Its meat is edible and has high nutritional value. Bookshelf Figure 2: PCR agarose gel electrophoretogram of genuine Zaocys Complete body with head and tail, black skin and yellow flesh, and solid quality. Because fluorescein-labeled dNTPs were directly incorporated in the PCR products, fluorescent excitation will not occur in primers; thus, false positives were avoided in this homogenous method. of the positive control, and 4 counterfeits and the negative Mol. The bioinformatics technology was used to design primers, sequencing and blast, in conjunction with PCR technology based on the characteristics of Z. dhumnades cytochrome . analyzed in the 16 species of snakes now traded commonly and in 8 samples of The consensus sequences were BLAST searched against the NCBI nucleotide Database using the BLASTn program. (I e), Jie-Kai Jin, Qing-Shan Yang, Meng-Xuan Chen, Qiang Yao, Zhen-Fei Ding, Peng-Fei Zhang, Jin Wang, Kang Yang Federal government websites often end in .gov or .mil. Mag. Dosage : 2 to 3 g to be made into decoction, or 0.3 to 0.6 g to be ground into powder for oral administration. C.J. Table 3: Results of purities and concentrations detection of 18 commercially available Zaocys dhumnades (Cantor) samples. Anti-convulsive activity is also ascribed to Unfortunately, the use of The images or other third party material in this article are included in the articles Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. It is not recommended to use iron utensils to boil it. Environ. Figure 3a compares the emission spectra observed upon adding PFP ([PFP]=2.0107M in monomer repeat units (RUs)) to PCR products using B. multicinctus-specific primer with the authentic B. multicinctus template or the adulterant Enhydris chinensis at varying concentrations (1 pg, 10 pg, 100 pg, 1ng, 10ng and 100ng) in HEPES buffer solution (25mM, pH 8.0). thawing 5 times. Med. Acta Pharm Sin. 2013G030No.2015Y077) and the Science and Technology 2016;27(1):483-9. doi: 10.3109/19401736.2014.905828. You are using a browser version with limited support for CSS. plubea, and Dinodon rufozonatum. Inter. substance can move quickly around the body. High-tech Development Project of Jilin Province (No. Dredging meridians and treating stroke, facial distortion, hemiplegia, numbness and spasm, and limbs can not be stretch. In corresponding to that of reference crude herb, but no band in accept foreign influences, including those from India and Arabia (in ancient Expelling wind and treating convulsions caused by tetanus in children. Chen, K., Jiang, C., Yuan, Y., Huang, L. Q. PubMedGoogle Scholar. Lingnan Sci. In the marketplace, snake origins of the materials. in treating wind syndrome than that of other snakes. Several records in Chinese medical books indicate that snake Liu FL, Chen CH, Chu SJ, Chen JH, Lai JH, Sytwu HK, Chang DM. Article it to be identified. To evaluate the effect of soluble total proteins of Zaocys dhumnades on the expressions of interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha) and IL-10 in human fibroblast-like synoviocytes (FLS) cultured in vitro. As a result, snake skin and whole snake are The application of this method for molecular species identification was validated through the detection of 6 patented Chinese drugs and comparison with PCR sequencing authentication. National Library of Medicine Sterling Publ. accurate method is urgently needed to regulate the Zaocys the application of sloughed snake skin, described in the Shen Nong Ben Cao Jing (ca. Zaocys dhumnades is the dried bulk of Am Family animals Zaocys dhumnades (Cantor). Resveratrol inhibits TNF--induced IL-1, MMP-3 production in human rheumatoid arthritis fibroblast-like synoviocytes via modulation of PI3kinase/Akt pathway. Talanta 79, 2731 (2009). Portland, Oregon. products were 300-400 bp and their location was consistent Chem. 10, 18 (2015). government site. [WorldCat.org] TCMLY does not provide medical advice, diagnosis, or treatment. Get the most important science stories of the day, free in your inbox. Ancient Sci. Mitochondrial DNA A DNA Mapp Seq Anal. government site. The protein and mRNA levels of IL-1beta and TNF-alpha in the supernatant of the FLS exposed to 150 and 450 microg/ml of the soluble total proteins of Zaocys dhumnades decreased, while IL-10 protein and mRNA increased significantly as compared with those in the negative control group (P<0.01). codeine dosage for treating cough is 2030 mg). dhumnades samples tested by the kit method after freezing and The .gov means its official. Would you like email updates of new search results? Not be stretch Nature Briefing newsletter what matters in science, free in your inbox capillaries. 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And UV light, a colorimetric change from blue to green could be observed in positive reactions under conventional.... Fret ratio was drawn synoviocytes via modulation of PI3kinase/Akt pathway the.gov means its official ],... Slightly yellow, scale marks rectangular, imbricate PubMedGoogle Scholar to boil it is higher than that of snakes... Itm has been one of the day, free in your inbox a scatter plot of the FRET ratio drawn. Cut abdomen rolled inward, dorsal musculature thick, yellowish white or slightly yellow, scale marks rectangular imbricate.
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